No!
In general 2-sample:-
1)common population variance:- if you know each datum in data is independent and Random Variable Normally distributed or you can apply CLT, then it is the Best option.
2)Pooled Variance:- Pooled variance is best estimate for population variance(first you'd accept equality of population variance of both separate data or you can use F-test to determine so). if you can accept equality of variance, then use Pooled variance.
3)separate sample variance:- to use this both sample size must be big enough, say, 20 or more/30 or more........ and each datum in data is independent and Random Variable Normally distributed but you can't accept equality of variance then use it.
For your case:-
Pooled Variance might be good if you collected data from same group(so that you can accept equality of variance) of individuals independently.
Last edited: Apr 27, 2015